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Image Search Results
Journal: Scientific Reports
Article Title: Fluorescence assay of dihydroorotate dehydrogenase that may become a cancer biomarker
doi: 10.1038/srep40670
Figure Lengend Snippet: ( a ) Principle of colourimetric assay for DHODH activity. ( b ) Absorbance change of DCPIP by enzyme reaction of endogenous DHODH in HeLa cells. The enzymatic reaction was performed at 37 °C for 0, 30 or 60 min in the presence of DCPIP, DHO and cell lysate. Absorbance of DCPIC in the reaction mixture was monitored at 610 nm. Data represent the mean ± SD of three separate experiments.
Article Snippet: Total protein concentration in tissue sample was measured using a
Techniques: Activity Assay